Italian guidelines to assess and manage the risk associated to bathing waters and recreational activities. It was discovered from french polynesia, new caledonia and the ryukyu islands, pacific ocean. Interannual variability in ostreopsis ovata bloom dynamic along genoa coast northwestern mediterranean. Ostreopsis ovata bloom along the conero riviera northern. The tropical genus ostreopsis has been recorded along italian coasts of the mediterranean sea since the 90s, but large bloom events have been reported only in recent years. New insights on cytological and metabolic features of ostreopsis cf. Here, we analyse sequence variability in ribosomal dna markers to provide the first phylogeographical study of. Feeding by heterotrophic protists on the toxic dinoflagellate. Although the large number of studies on this dinoflagellate, the mechanisms underpinning o.
Eurosurveillance ostreopsis ovata algal blooms affecting. Three ostreopsis species have been recorded so far in various mediterranean regions. Respiratory illness as a reaction to tropical algal blooms occurring in a temperate climate to the editor. Cultivated as a house plant in europe and america for over a hundred years. The results demonstrate the effectiveness of the innovative automatic method for ostreopsis ovata identification and counting. Life cycle stages of the benthic palytoxinproducing. Modelling growth dynamics and toxin production in ostreopsis. The presence of the potentially toxic genera ostreopsis. This species is primarily an epiphytic benthic dinoflagellate, but it also lives in the water column and sometimes forms harmful red tides. Per approfondimenti rivolgersi al gruppo di ricerca di ecologia della colonna dacqua del centro for more info refer to the research group of ecology of the watercolumn of the centre ostreopsis. Lcms studies have identified a number of palytoxinlike compounds, termed ovatoxins, along with trace amounts of putative palytoxin as the causative agents of the o. Members of the species may produce toxins that contribute to ciguatera fish poisoning. Ostreopsis ovata is an epiphytic potentially toxic dinoflagellate. Three strains of ostreopsis ovata were isolated from sea water and algae collected either along the tyrrhenian or the adriatic sea.
Jade plant, crassula ovata crassula ovata is a common houseplant that is usually called jade plant, or less frequently referred to as friendship plant, money plant, or silver dollar plant. In each bottle, the final ostreopsis prey concentration was ca. Despite many studies, important biological features of this species are still poorly known. Codonopsis ovata seeds glosocomia ovata, wahlenbergia. Massive occurrence of the harmful benthic dinoflagellate. Pdf three strains of ostreopsis ovata were isolated from sea water and algae collected either along the tyrrhenian. Read occurrence of ostreopsis ovata dinophyceae in the tyrrhenian sea during summer 1994, botanica marina on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Isolation and structure elucidation of ovatoxina, the. It has a worldwide distribution, normally associated with other epiphytic or benthic dinoflagellates.
Genetic diversity of ostreopsis ovata dinophyceae from malaysia. Transcriptomic investigation into polyketide toxin synthesis. Interannual variability in ostreopsis ovata bloom dynamic. Ostreopsis ovata cell densities increased at all light intensities except at 10 mol m2 s1. Physiological responses of ostreopsis ovata to changes in n. Such an alga has caused severe sanitary emergencies and economic losses due to its production of palytoxinlike compounds. We tested the hypothesis that as it is benthic, it would show distinct biogeographical patterns in comparison with planktonic species. Comparative growth of three strains of ostreopsis ovata at. Isolates were characterized morphologically based on lm and sem, and phylogenetic analyses were conducted by sequencing the lsu rdna d1d3, its. In the summers of 2003 and 2004, 2 microalgal blooms of potentially toxic dinoflagellates genus ostreopsis, usually distributed in tropical waters occurred along the coasts of bari, south italy. Aim ostreopsis is a benthic and epiphytic dinoflagellate producing potent toxins widespread in tropical and warm temperate coastal areas world. The aim of this study was to improve understanding of the ecophysiology of ostreopsis cf.
All the samples have been counted both by traditional optical microscopy at each arpa and by automatic optoelectronic method at cnribf in genoa. Codonopsis ovata seeds glosocomia ovata, wahlenbergia roylei, kashmir bellflower plant world seeds. Nov 22, 2011 in this article we report on the liquid chromatography tandem mass spectrometry lcms investigation of plankton samples collected in the summer of 2006 along the ligurian coasts, coinciding with a massive bloom of the tropical microalga ostreopsis ovata. We report the development and validation of a qpcr based method for estimation of the toxic benthic dinoflagellate ostreopsis cf. Furthermore, this unicellular alga has a biconvex theca with the hypotheca. Detailed studies of the inner face of the thecal wall of ostreopsis. Background we describe the development and validation of a new quantitative real time pcr qrtpcr method for the enumeration of the toxic benthic dinoflagellate ostreopsis cf. Mba testing was based on two different extraction protocols, while hna also. In this study, two species have been isolated and cultured, ostreopsis ovata and ostreopsis lenticularis. Lcms analyses indicated the occurrence of putative palytoxin along with a much more abundant palytoxinlike compound never reported so far. An integrated study, using different microscopy and molecular techniques, raman microspectroscopy and. Pdf comparative growth of three strains of ostreopsis ovata at. However, purified ovatoxins are not widely available and their toxicities are still unknown.
New insights on cytological and metabolic features of. So far, any risk assessment for ovatoxins as well as. Results indicated that under nutrient sufficiency o. In the last decades, the italian coastlines have been plagued by the recurring presence of the benthic dinoflagellate ostreopsis ovata. Respiratory illness as a reaction to tropical algal blooms. Generally, it is a very easy and productive plant to grow, provided its needs are understood and met. Eurosurveillance ostreopsis ovata and human health. Lcms analyses indicated the occurrence of putative palytoxin along with a much more abundant palytoxinlike compound never.
Evaluation of the automatic identification and counting. New approach using the realtime pcr method for estimation. This item has no pdf download ostreopsis ovata algal blooms affecting human health in genova, italy, 2005 and 2006 c brescianini, c grillo, n melchiorre, r bertolotto, a ferrari, b vivaldi, g icardi, l gramaccioni, e funari, s scardala. Isolation and structure elucidation of ovatoxina, the major. New approach using the realtime pcr method for estimation of. Since, in coordination with several regione liguria institutions, cima research foundation researches and develops the. Feeding by heterotrophic protists on the toxic dinoflagellate ostreopsis cf.
Species in this genus are anterioposteriorly compressed and are observed in apical or antapical view. While this study focuses only on respiratory disorders, the outcomes of this research can contribute to the assessment of the general risks to the public health and the environment posed by. Genetic diversity of ostreopsis ovata dinophyceae from. In order to investigate the toxicity of ostreopsis species present in greek coastal waters, cultures of ostreopsis sp. Physiological responses of ostreopsis ovata to changes in n and p availability and temperature increase nayani k.
This item has no pdf download ostreopsis ovata and human health. Morphology and molecular phylogeny of ostreopsis cf. The morphological differences of all three ostreopsis spp. Since 2005, the benthic dinoflagellate ostreopsis cf. The presence of the potentially toxic genera ostreopsis and. Crassula ovata, crassula argentea, crassula portulacea. Aug 15, 2019 to investigate the genetic basis of pltx. Quantification of the toxic dinoflagellate ostreopsis spp. Crassulaceae jade plant, dollar plant, money tree origin.
The genus ostreopsis contains benthic and potentially toxic species that are distributed in tropical and temperate regions. This beautiful specimen is found in the himalayas, from pakistan to kashmir, at altitudes of 30004200m and. Gladan 1, jasna arapov 1, silvia casabianca 2,3, antonella penna 2,3, giorgio honsell 4, valentina brovedani 5, marco pelin 5, luciana tartaglione 3,6, silvio sosa 5, carmela dellaversano 3,6, aurelia tubaro 5, ante zuljevic. Ostreopsis is a genus of freeliving dinoflagellates found in marine environments.
Toxic events in humans after inhalation or cutaneous contact have been reported during o. Jade plant, crassula ovata master gardener program. This work identifies the first occurrence of ostreopsis with the morphology and phylogeny of hainan strains in chinese waters. Ostreopsis, mediterranean sea, benthic dinoflagellates, harmful algae, cyst. Ostreopsis ovata bloom along the conero riviera northern adriatic sea. In this article we report on the liquid chromatography tandem mass spectrometry lcms investigation of plankton samples collected in the summer of 2006 along the ligurian coasts, coinciding with a massive bloom of the tropical microalga ostreopsis ovata.
Complex toxin profile of french mediterranean ostreopsis cf. Download pdf 82 kb abstract species belong to the genus ostreopsis schmidt, all of these are toxic, and ostreopsis ovata fukuyo is one of these. Ecology of the epiphytic potentially harmful dinoflagellate. Grosseto, tuscany, italy, issn 07490208in the last years ostreopsis spp. Health problems after ostreopsis aereosol exposure in italy. Other species of ostreopsis were found, but they turned out to be restricted to just one of the two main warm. In this study, we present the first report of observations of ostreopsis cf. Complex toxin profile of french mediterranean ostreopsis. Ostreopsis ovata, gambierdiscus toxicus, coolia monotis and other benthic dinoflagellates are known to produce copious amounts of mucilage, which has a role in the attachment of these species to surfaces with the formation of transparent films embedding many cells.
Physiological responses of ostreopsis ovata to changes in. The true kashmir bellflower is a gorgeous plant with slender erect stems bearing large, single, pendulous, milkyblue bellshaped flowers which are a noticeable flared tentshape. Relationships with environmental conditions and substrata. A phylogeographical study of the toxic benthic dinoflagellate. Transcriptomic investigation into polyketide toxin. The genus ostreopsis is an important component of benthic and epiphytic dinoflagellate assemblages in coral reefs and seaweed beds of malaysia.
Introduction under the light microscope ostreopsis ovata appears flat and tearlike shaped. Crassula ovata, crassula argentea, crassula portulacea, crassula obliqua family. Jun 15, 2017 physiological responses of ostreopsis ovata to changes in n and p availability and temperature increase nayani k. Phylogeny, toxin profile and contamination of mussels and marine plastic litter article pdf available in toxins 118 july 2019 with 274 reads.
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